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A Novel Application of Pineapple Core Extract: In Vitro Antibacterial Activity Against Staphylococcus aureus and Streptococcus pyogenes from Impetigo Lesions

Year: 2026

Type: article

Abstract: Background Impetigo is a superficial bacterial skin infection primarily caused by Staphylococcus aureus and Streptococcus pyogenes. The pineapple core (Ananas comosus L. Merr. var. Queen) has been reported to contain various bioactive compounds, including bromelain and flavonoids, which possess antibacterial properties effective against Staphylococcus aureus. This study aimed to compare the susceptibility of Staphylococcus aureus and Streptococcus pyogenes to fusidic acid and pineapple core extract (PCE) in patients diagnosed with impetigo. Methods An in vitro experimental study using a post-test only control group design, conducted between January and March 2025. A total of 60 bacterial isolates of Staphylococcus aureus and Streptococcus pyogenes were collected from patients with impetigo who met the inclusion criteria. The isolates were divided into five treatment groups, with each bacterial group receiving interventions in concentrations of 50%, 70%, and 100% PCE, 10 μg fusidic acid (positive control), and a negative control. The inhibition zone diameters were measured using the disk diffusion method. Results There was a significant difference in susceptibility of Staphylococcus aureus to the 50%, 70%, and 100% concentrations of PCE compared to fusidic acid. However, Streptococcus pyogenes showed no statistically significant difference in susceptibility between the 100% pineapple extract and 10 μg fusidic acid. The descending order of inhibition zone diameter observed in both bacteria was as follows: 10 μg fusidic acid, 100% PCE, 70% PCE, 50% PCE, and negative control. Conclusion Streptococcus pyogenes isolates from impetigo patients demonstrated susceptibility to both 100% PCE and fusidic acid, indicating the potential of PCE as a natural antibacterial agent

Source: F1000Research

DOI: 10.12688/f1000research.171024.1