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Measurement of respiration in ex vivo mitochondria isolated from fresh human brain

Year: 2026
Type: article
Abstract: Thirty-two pediatric craniotomies provided fresh brain tissue. Respiratory measurements of partially and Ficoll gradient purified mitochondria was optimized using the Seahorse XFe96 Analyzer. RNAseq verified differences. 25µg of partially purified mitochondria isolated with a 2.5% EGTA respiratory buffer at pH 7.4 provided optimal measurements. Tumor tissue had very low respiration. Some epileptic foci exhibited higher respiratory rates while others exhibited lower respiratory rates relative to surrounding non-seizure tissue. RNAseq analysis revealed distinct gene expression patterns between these two groups. This study demonstrates, for the first time, that mitochondrial respiration can be reliably measured from fresh, ex vivo human brain tissue.
DOI: 10.1016/j.brainres.2026.150156
Authors: Patrick J. McCarty, Brianna A. Werner, Adrienne C. Scheck, Eric Wang, Indrapal N. Singh, John Gaitanis, P. David Adelson, Richard E. Frye
Institutions: The University of Texas at San Antonio Health Science Center, Creighton University, University of Arizona College of Medicine- Phoenix, Midwestern University, Brown University, Hasbro Children's Hospital, West Virginia University
Language: English
Open Access status: hybrid
Estimated APC paid: $3,360
OpenAlex ID: W7118480512
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Cites: 89
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Related to: 9