Work

Effect of low-dose gamma irradiation on seed-borne transmission of tomato brown rugose fruit virus in tomato

Year: 2026

Type: article

Abstract: Tomato brown rugose fruit virus (ToBRFV), a highly virulent tobamovirus, poses a major threat to global tomato production by overcoming host resistance and traditional control measures. This study evaluates the efficacy of low-dose gamma irradiation (10, 15, and 20 Gy) in reducing ToBRFV contamination in tomato seeds. Contaminated seeds were irradiated and assessed for germination rate, chlorophyll content, stem diameter, and viral accumulation with RT-qPCR. The potential synergistic effect of combining 15 Gy gamma irradiation with 2.5 % sodium hypochlorite (NaOCl) was also investigated. Results revealed that 15 Gy significantly improved germination, enhanced chlorophyll levels, and increased stem thickness, while substantially reducing viral replication. In contrast, 20 Gy had detrimental effects on both plant growth and viral suppression. The combination of 15 Gy and NaOCl further decreased viral accumulation, though at the cost of reduced germination rates. Applying 15 Gy confers dual benefits, including effective seed disinfection and improved host resistance. It shows strong potential for use in integrated tomato disease management in greenhouse and field conditions.

Source: Journal of Genetic Engineering and Biotechnology

DOI: 10.1016/j.jgeb.2025.100644

Authors: Kimia Tokhmechi, Abozar Ghorbani, Davoud Koolivand, Mahsa Rostami, Nahid Hajiloo

Institutions: University of Zanjan, Atomic Energy Organization of Iran, Nuclear Science and Technology Research Institute

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: gold

Estimated APC paid: $1,000