Mapping of QTL for adult plant stripe rust resistance genes in a Sichuan wheat landrace and the evaluation of their breeding effects
Year: 2024
Type: article
Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a worldwide wheat disease that causes large losses in production. The lack of in-depth understanding of genetic and breeding utilization effects, coupled with the linkage of undesirable traits, are the key factors that limit the wide application of most of the discovered wheat stripe rust resistance genes in breeding and production. Our previous studies have shown that the wheat landraces, Hongmangmaizi (HM), exhibited stable adult plant stripe rust resistance to prevalent physiological races and pathogenic groups of stripe rust in China. In order to reveal the genetic basis of HM adult plant resistance to stripe rust, F1, F2, and F2:3 segregating populations derived from the cross between Avocet S and HM were inoculated with mixed Pst and evaluated for stripe rust reaction at adult stage in the field. Through bulked segregation analyses (BSA) using 55K single-nucleotide polymorphism array and exon sequencing technology, we identified two quantitative trait loci (QTL) on chromosomes 7AL and 7DS, designated as QYr.HM-7AL and QYr.HM-7DS, explaining 11.64%-15.25% and 24.33%-40.58% of the phenotypic variance, respectively. Through integrated linkage, genetic and physical map analyses indicated that the major QTL QYr.HM-7DS was corresponding to the Yr18 gene, whereas QYr.HM-7AL, which was the second most stable QTL, should be a potential novel QTL for adult-stage stripe rust resistance. Furthermore, the kompetitive allele- specific PCR (KASP) markers, tightly linked with QYr.HM-7AL, were developed and validated. Based on construction of BC1F2 genetic improvement population derived from a cross between Mianmai 1618 × HM, the main QTL genetic effect of stripe rust resistance and its synergistic effect on yield related traits were analyzed. The results showed that the two QTL (Yr18 and QYr.HM-7AL) could significantly reduce the damage of stripe rust and had a positive effect on spike length and tillering number under the genetic background of Mianmai 1618. The above results indicated that the QTLs of adult-stage stripe rust resistance from HM could be used in wheat yield breeding programs.
Source: ACTA AGRONOMICA SINICA
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $941
OpenAlex ID: W7118734829