Work

Nitrogen‐Doped Carbon Dots‐Eu 3+ ‐Tetracycline as a Ratiometric Fluorescence Probe for Turn “Off–On” Determination of Pyrophosphate and Alkaline Phosphatase Activity

Year: 2026

Type: article

Abstract: ABSTRACT The monitoring of alkaline phosphatase (ALP) activity and pyrophosphate (PPi) concentration is critical for assessing phosphorus dynamics and eutrophication in aquatic ecosystems. However, on‐site, rapid detection of these dual targets in complex water matrices remains a challenge. In this work, a nitrogen‐doped carbon dots (N‐CDs)‐Eu 3+ ‐tetracycline (TC)‐based ratiometric fluorescence probe was constructed for turn “off–on” determination of PPi and ALP based on the strong binding interaction between PPi and Eu 3+ and PPi hydrolyzed by ALP. In the N‐CDs‐Eu 3 + ‐TC system, with the addition of PPi, PPi could replace the N‐CDs to form the PPi‐Eu 3+ ‐TC multicomplex, and N‐CDs were released, resulting in the fluorescence quenching of Eu 3+ and fluorescence recovery of N‐CDs. ALP could hydrolyze PPi to PO 4 3− , which could destroy the PPi‐Eu 3+ ‐TC multicomplex and form the N‐CDs‐Eu 3+ ‐TC multicomplex again, and the fluorescence of Eu 3+ at 617 nm was recovered. The N‐CDs‐Eu 3+ ‐TC‐based ratiometric fluorescent probe possesses a quick fluorescence response, obvious fluorescence color variance, and excellent selectivity and sensitivity with LOD of 40 nM for PPi and 0.03 U/L for ALP. The proposed probe was successfully applied for the detection of PPi and ALP in the actual environmental water samples or serum samples.

Source: Luminescence

DOI: 10.1002/bio.70417

Authors: Shengnan Yin, Changlun Tong

Institution: Zhejiang University

Language: English

Topic: Carbon and Quantum Dots Applications

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

SDG: Clean water and sanitation

Open Access status: bronze

Funders: National Natural Science Foundation of China, Zhejiang University, Natural Science Foundation of Zhejiang Province