Work

Protocol for developing a mucus-producing gut-on-a-chip model from Caco-2 and HT29-MTX-E12 cells

Year: 2026

Type: article

Abstract: Organ-on-chip (OoC) technology offers a more relevant gut model in comparison with static in vitro gut models from stable cell lines by replicating the 3D structure, mucus production, fluid dynamics, and mechanical forces, closely simulating in vivo conditions. Here, we present a protocol for developing a mucus-producing gut-on-a-chip model from Caco-2 and HT29-MTX-E12 cells. We describe steps for assessing model viability, evaluating barrier integrity, and employing different microscopy techniques, including confocal microscopy.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104321

Authors: Valerija Movcana, Vendija Kozlova, Arnis Strods, Felikss Rumnieks, Justs Zalums, Arturs Abols

Institution: Latvian Biomedical Research and Study Centre

Language: English

Topic: 3D Printing in Biomedical Research

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550