Solid–Liquid Flow Analysis Using Simultaneous Two-Phase PIV in a Stirred Tank Bioreactor
Year: 2026
Type: article
Abstract: Solid–liquid stirred tanks are widely used in multiphase processes, including bioreactors for mesenchymal stem cell (MSC) culture, yet simultaneous experimental data for both dispersed and carrier phases remain limited. Here, a refractive index-matched (RIM) suspension of PMMA microparticles (dp=168μm, ρp/ρl≈0.96) in an NH4SCN solution is studied at an intermediate Reynolds number (Re≈5000), low Stokes number (St=0.078), and particle volume fractions 0.1≤αp≤0.5 v%. This system was previously established and studied for the effect of addition of particles on the carrier phase. In this work, a dual-camera PIV set-up provides simultaneous velocity fields of the liquid and particle phases in a stirred tank equipped with a three-blade down-pumping HTPGD impeller. The liquid mean flow and circulation loop remained essentially unchanged with particle loading, whereas particle mean velocities were lower than single-phase and liquid-phase values in the impeller discharge. Turbulence levels diverged between phases: liquid-phase turbulent kinetic energy (TKE) in the impeller region increased modestly with αp, while solid-phase TKE was attenuated. Slip velocity maps showed that particles lagged the fluid in the impeller jet and deviated faster from the wall in the upward flow, with slip magnitudes increasing with αp. An approximate axial force balance indicated that drag dominates over lift in the impeller and wall regions, while the balance is approximately satisfied in the tank bulk, providing an experimental benchmark for refining drag and lift models in this class of stirred tanks.
Source: Fluids
Institution: University of Liège
Language: English
Topic: Fluid Dynamics and Mixing
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,172
OpenAlex ID: W7119191822