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Turbo-RIP: A Protocol for TurboID-based RNA Immunopurification to Map RNA Landscapes in Plant Biomolecular Condensates

Year: 2026

Type: article

Abstract: , validation of TurboID activity, and RNA recovery. The protocol successfully captured processing body-associated RNAs with minimal background. Turbo-RIP enables systematic mapping of RNA populations within plant condensates under diverse conditions. The protocol requires 3-5 days from sample preparation to RNA isolation, with construct validation taking 2-4 weeks. All procedures use standard laboratory equipment, making Turbo-RIP accessible for plant molecular biology laboratories. Key features • Apply TurboID-based proximity labeling specifically for RNA capture in plant condensates. • Optimize experimental conditions for different plant species and condensate types. • Implement quality control measures and data analysis pipelines.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5587

Authors: Zhi Zhang, Yanting Xu, Hanxiang Liu, Chen Liu, Panagiotis Moschou

Institutions: Sun Yat-sen University, University of Crete, Swedish University of Agricultural Sciences, FORTH Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas

Language: English

Topic: Biotin and Related Studies

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W7119521782

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