Investigação do comportamento de tolerância ao N, N- dietilmetilbenzamida (DEET) em populações de Aedes aegypti (Diptera: Culicidae)
Year: 2020
Type: dissertation
Abstract: Aedes aegypti is a mosquito of great interest for public health, as it is the vector of arboviruses, such as dengue, chikungunya and Zika.Biological, mechanical and chemical control, with the use of insecticides and repellents, are used against this vector.Repellents are considered one of the best ways to avoid female bites, being those that have N, Ndiethylmethylbenzamide (DEET) in the formula the most commercialized, as well as the most studied today.However, resistance to some insecticides such as pyrethroid, can lead to changes in the locomotor activity of mosquitoes.Thus, this study aims to evaluate aspects related to DEET tolerance and resistance to xenobiotics in field Ae. aegypti (Laranjeiras) and laboratory (Rockefeller) strains.Repellency tests were performed with Ae. aegypti for the response to human odor.In each test, 50 females were used, totaling 700 females from the Laranjeiras population and 1500 females from the Rockefeller population.The females were placed in test cages for about two hours in order to adapt to the environment.Subsequently, the repellency test was performed, which consisted of exposing the mosquito to 0.5 mL of DEET (10% in ethanol), which was applied evenly to the human forearm.To diagnose the presence of the kdr mutation in the population of Laranjeiras, 32 females from that population were analyzed.To evaluate the locomotor activity of Ae. aegypti Rockefeller and Laranjeiras females,, the Drosophila Activity Monitor -Trikinetics was used.In the period from September to November 2019, 290 ovitraps were installed in the municipality of Laranjeiras -SE, with a total of 80.3% of reeds with the presence of Aedes eggs, accounting for 15,940 eggs.After conducting the bioassays, it was possible to observe greater sensitivity to DEET in females from the field.In addition, it was also possible to observe the presence of the kdr mutation and greater locomotor activity in this population.Thus, our results suggest that resistant mosquitoes may be more susceptible to DEET.
Author: Pollyana Conceição Romão Maia
Language: Portuguese
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3122777906