Year: 2026
Type: article
Abstract: Chronic kidney disease (CKD) is associated with systemic complications, including pulmonary alterations due to kidney–lung crosstalk. This study evaluates Ki-67 immunohistochemical expression in rat lung tissue to assess cellular proliferative activity under experimental chronic renal failure (CRF) conditions, considering age-related variations. Outbred male rats (n = 150, aged 6, 9, and 12 months) were divided into control and experimental groups. CRF was induced via repeated intramuscular injections of 50% glycerol (0.5 mL twice weekly for 1 month). Alveolar lung tissues were fixed, sectioned, and stained for Ki-67 using the 3,3′-Diaminobenzidine method. Quantitative analysis was performed with QuPath software (v0.4.0) to calculate proliferative indices. Data were analyzed using two-way ANOVA with post-hoc tests (p <0.05). In controls, Ki-67 proliferative indices declined with age: 55.86% at 6 months, 52.32% at 9 months, and 23.44% at 12 months. Under CRF, indices were significantly suppressed: 21.75, 11.06, and 2.32%, respectively (p <0.01 vs. controls), indicating impaired cellular renewal. Experimental CRF exacerbates age-dependent reductions in lung cell proliferation, highlighting mechanisms, such as inflammation and hypoxia in pulmonary pathology. These findings underscore the need for targeted interventions in CKD-related lung dysfunction.
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W7123340910