Protocol for the injection of adenoviruses into brown adipose tissue in mice
Year: 2026
Type: article
Abstract: Brown adipose tissue (BAT) represents a specialized form of adipose tissue that has attracted much attention in the search for therapeutic interventions to treat obesity and its related metabolic disorders. Here, we present a protocol to modulate BAT metabolism in vivo in mice via the direct administration of adenovirus (Ad). We also describe steps for the delivery of postoperative care, including the administration of analgesics and the application of antiseptics to the surgical site to prevent potential infections. For complete details on the use and execution of this protocol, please refer to Al-Massadi et al. 1 • Instructions for performing injections of adenoviruses into the BAT • Steps to genetically manipulate BAT cells • Guidance and considerations for the metabolic regulation of BAT Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Brown adipose tissue (BAT) represents a specialized form of adipose tissue that has attracted much attention in the search for therapeutic interventions to treat obesity and its related metabolic disorders. Here, we present a protocol to modulate BAT metabolism in vivo in mice via the direct administration of adenovirus (Ad). We also describe steps for the delivery of postoperative care, including the administration of analgesics and the application of antiseptics to the surgical site to prevent potential infections.
Source: STAR Protocols
Institutions: Complejo Hospitalario Universitario de Santiago, Centro de Investigación Biomédica en Red, Instituto de Investigación Sanitaria de Santiago, Servicio Gallego de Salud, Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition, Universidade de Santiago de Compostela
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
Awards: G1496096456, Búsqueda de nuevos biomarcadores para el diagnóstico de obesidad y el déficit cognitivo asociado, Las vesículas extracelulares como mediadoras de la comunicación entre órganos en la obesidad: dianas innovadoras y plataforma traslacional de biomarcadores vesiculares, G443141326, Biomarcadores de reprogramación metabólica asociados al efecto protector de la lactancia materna sobre la obesidad y patologías asociadas., Papel de la hormona asprosin en las enfermedades hepáticas
OpenAlex ID: W7123345315