Single-cell and spatial transcriptomic profiling of cardiac fibroblasts following myocardial infarction
Year: 2026
Type: data-paper
Abstract: Cardiac fibroblasts (CFs) are key mediators of heart repair following myocardial infarction (MI). A specific CF subpopulation, termed Reparative Cardiac Fibroblasts (RCFs), has been shown to orchestrate scar formation and prevent ventricular rupture after MI. However, the timing of RCF appearance and the molecular events underlying this transition remain largely undefined. Here, we present a multi-modal dataset capturing the transcriptional dynamics of CFs during the early phase post-MI. Our integrative dataset combines bulk RNA sequencing, RNAscope in situ hybridization, and spatial transcriptomics to anatomically and temporally map the gene expression changes associated with the transition into RCFs. The dataset provides resources to characterize the distinct molecular programs that guide the emergence of RCFs from Periostin ( Postn ) + activated CFs. This dataset provides a valuable resource for investigating CF heterogeneity and reparative pathways following MI. All raw and processed data, along with detailed metadata and annotations, are made available to facilitate reuse by the cardiovascular and single-cell biology communities.
Source: Scientific Data
Authors: Silvia C. Hernández, Marina Ainciburu, Laura Sudupe, Nuria Planell, María López-Moreno, Amaia Vilas-Zornoza, Luis Diaz-Martinez, Jorge Cobos-Figueroa, Juan P. Romero, Sarai Sarvide, Patxi San Martin-Uriz, Ana López-Pérez, Gloria Abizanda, Purificación Ripalda-Cemboráin, Emma Muinos-López, Vincenzo Lagani, Jesper Tegner, Ming Wu, Stefan Janssens, José M. Pérez-Pomares, Felipe Prósper, Adrián Ruiz-Villalba, David Gómez-Cabrero
Institutions: Albert Einstein College of Medicine, Navarre Institute of Health Research, Clinica Universidad de Navarra, Centro de Investigación Médica Aplicada, King Abdullah University of Science and Technology, Andalusian Centre for Nanomedicine and Biotechnology, Instituto de Investigación Biomédica de Málaga, Universidad de Málaga, Centro de Investigación Biomédica en Red de Cáncer, Universidad Pública de Navarra (UPNA), Navarrabiomed, Ilia State University, Karolinska University Hospital, Science for Life Laboratory, Karolinska Institutet, KU Leuven
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,490
OpenAlex ID: W7123353232