Improvement of the Culture Medium for Latilactobacillus sakei Exhibiting High Lactic Acid Production Isolated Using Response Surface Methodology
Year: 2026
Type: article
Abstract: Lactic acid is a crucial bio-based chemical with widespread applications in industries such as the food, chemical, bioplastic, and pharmaceutical industries. As demand for lactic acid rises, the search for efficient fermentation strains has become increasingly important. This study aimed to optimize fermentation conditions to enhance lactic acid production using Latilactobacillus sakei L-7. We began by screening key medium components and process parameters through single-factor experiments. Subsequently, we applied response surface methodology for a more comprehensive optimization. The optimal medium formulation was determined to be 40 g/L glucose, 39.54 g/L yeast extract, 10 g/L CH3COONa, 6 g/L K2HPO4, 0.2 g/L MnSO4, 0.4 g/L MgSO4, and 1 mL/L Tween 80. Under the optimized fermentation conditions of 30.27 °C and pH 8.46, the lactic acid production reached 26.18 ± 0.44 g/L, a 50.6% increase compared to pre-optimization levels. These results offered preliminary support for utilizing L. sakei L-7 in the industrial production of lactic acid.
Source: Processes
DOI: 10.3390/pr14020283
Authors: Zhirong Zhang, Wanjing Hong, Min Xu, Zhongkai Yi, Xu Yao, Zhijiang Zhou, Ye Han
Institution: Tianjin University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,896
OpenAlex ID: W7124134438