Work

Alcohol Oxidase–Imine Reductase Cascade for One-Pot Chiral Amine Synthesis

Year: 2026

Type: article

Abstract: We report a one-pot biocatalytic cascade for the conversion of allylic alcohols into enantioenriched secondary amines through a sequence involving oxidation followed by conjugate reduction-reductive amination (CR-RA). This transformation, catalyzed by a cholesterol oxidase (ShCO) and an ene-imine reductase (EneIRED), proceeds under mild conditions and accommodates a broad substrate scope. In several cases, the cascade outperforms the stepwise method and exhibits substrate-dependent selectivity, highlighting the advantages of tandem enzymatic systems for efficient and streamlined chiral amine synthesis.

Source: ACS Catalysis

DOI: 10.1021/acscatal.5c06313

Authors: Anya Miletic, Christopher J. Truby, Nicholas J. Turner, Rebecca E. Ruscoe

Institutions: University of Manchester, Keele University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: Biochemical Society, Royal Society of Chemistry, Engineering and Physical Sciences Research Council