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Inhibitory effects of water chestnut (Trapa bispinosa Roxb.) extract plus lutein on the progression of cataract and their impact on the retina and aorta in diabetic rats

Year: 2026

Type: article

Abstract: Trapa bispinosa Roxb. extract (TBE), combined with lutein, has an inhibitory effect on cataract progression. However, no prior studies evaluated oxidative stress- and antioxidant-related factors simultaneously. This study aimed to investigate TBE plus lutein’s potential mechanism to suppress cataract progression and examine its effects on retinal tissue and aorta in spontaneous diabetic Torii (SDT) rats. Seven-week-old SDT rats ( n = 10) and Sprague-Dawley (SD) control rats ( n = 5) were divided into three groups: SDT-administered TBE plus lutein (TBE plus lutein), untreated SDT, and control. Blood glucose levels and anterior segment photographs were obtained. Subsequently, polymerase chain reaction (PCR) was used to measure the expression of oxidative stress- and antioxidant-related genes in the lenses, retinas, and aortas. Immunohistochemical staining of the lenses was performed to detect the expression of NOX-1 (an oxidative stress-related factor), 4-HNE (a lipid peroxidation product), and CEL (an advanced glycation end product [AGE]). The progression of diabetic cataract was suppressed in the TBE plus lutein group. PCR results revealed that NOX-1 in lenses was significantly suppressed in the TBE plus lutein group ( p < 0.05), whereas antioxidant-related factors, such as SOD, GPX, and Peroxiredoxin 6, were significantly increased ( p < 0.01). A similar trend in NOX-1 expression was observed in the retinas and aortas, but the difference was not significant. Immunohistochemical staining of lenses revealed reduced NOX-1, 4-HNE, and CEL expression in the lenses of the TBE plus lutein group. TBE plus lutein may inhibit the progression of diabetic cataract by increasing antioxidant-related factors rather than suppressing oxidative stress-related factors.

Source: Journal of Diabetes & Metabolic Disorders

DOI: 10.1007/s40200-025-01845-9

Authors: Hiroshi Mizuno, Gaku Ishigooka, Denan Jin, Shinji Takai, Teruyo Kida

Institution: Osaka Medical and Pharmaceutical University

Language: English

Topic: Natural Antidiabetic Agents Studies

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,990