Work

Single-atom Pt doped nanoceria for enhanced cell phagocytosis and nanozyme activities in keratitis immune regulation

Year: 2026

Type: article

Abstract: During keratitis treatment, oxidative stress and inflammation often result in corneal neovascularisation, scarring, and reduced light transmittance. In this study, single-atom Pt/CeO 2 is synthesised, exhibiting significantly enhanced catalase-like and superoxide dismutase-like activities for the elimination of superoxide anions (•O 2 − ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radicals (•OH). Doping single-atom Pt onto CeO 2 increases the Ce 3+ concentration in the Ce 3+ /Ce 4+ ratio from 39.12% to 58.66%, as confirmed by electron spin resonance, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. In vitro studies demonstrate that single-atom Pt/CeO 2 effectively reduces intracellular ROS levels in H 2 O 2 -activated human corneal epithelial cells. Additionally, it exerts an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages, significantly decreasing the expression of interleukin-1β, interleukin-6, and tumour necrosis factor-α. In vivo , in an LPS-induced keratitis animal model, single-atom Pt/CeO 2 accelerates corneal ulcer healing and preserves corneal light transmittance, attributed to its anti-inflammatory properties, enzyme-like activities, and ability to promote cell migration. This study offers a novel approach for treating various inflammatory and autoimmune diseases.

Source: Journal of Nanobiotechnology

DOI: 10.1186/s12951-025-03869-y