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PLGA nanoparticles restore acidic pH and degradative function to compromised lysosomes with Cy3-labeling providing enhanced tracking to lysosomes

Year: 2026

Type: article

Abstract: Tools that restore acidic pH in compromised lysosomes can enhance autophagy and waste clearance in degenerative disorders characterized by excessive accumulation. Here, we describe the synthesis of lysosome-targeted nanoparticles composed of poly(d,l-lactide-co-glycolide) (PLGA) polymers covalently bound to the fluorescent dye Cyanine3 amine (Cy3). These Cy3-PLGA nanoparticles enable precise tracking of lysosomal delivery and demonstrate sustained long-term retention within lysosomes, supporting their potential for future applications aimed at restoring lysosomal pH in aging and degenerating diseases.

Source: American Journal of Physiology-Cell Physiology

DOI: 10.1152/ajpcell.00494.2025

Authors: Jiaqi Li, Tianchen Wang, Wennan Lu, Davit Jishkariani, Andrew Tsourkas, Simon Kaja, Kyle H. Vining, Jedtanut Thussananutiyakul, Ashley Spence, Rohini M. Nair, Joshua L. Dunaief, Claire H. Mitchell

Institutions: University of Pennsylvania, Loyola University Chicago

Language: English

Topic: Nanoparticle-Based Drug Delivery

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

OpenAlex ID: W7124178644

FWCI: 2.835

Cites: 76

Cited by: 2

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