Work

Hypatia: Comparative Isoform Profiling Across Cell Populations from Long-Read Single-Cell Transcriptomes

Year: 2026

Type: preprint

Abstract: High-throughput long-read single-cell RNA-sequencing enables isoform-level study across single cells, yet methods for systematically assessing cell-to-cell variations remain limited. Here, we develop Hypatia, a comprehensive platform for dissecting isoform complexities across cell populations, devising Tsallis entropy and Cramer's V to facilitate robust comparative profiling. Hypatia revealed prominent isoform species variations and usage shifts across cell-types in glioblastoma, renal cell carcinoma, and heart, highlighting clinically relevant applications for studying isoform-derived, cell-specific functions.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.01.13.699341

Authors: Timothy Pan, Cheng-Kai Shiau, Lina Lu, Changhu Wang, Minhua Wang, Yueying He, Arvind Bhimaraj, Daniel Brat, Jason Huse, Jingyi Jessica Li, Ruli Gao

Institutions: Northwestern University, Robert H. Lurie Comprehensive Cancer Center of Northwestern University, University of Washington, Fred Hutch Cancer Center, Houston Methodist, The University of Texas MD Anderson Cancer Center

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G3930547285

OpenAlex ID: W7124264354

Cites: 18

Cited by: 1

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