Work

VAPA at the inner nuclear membrane affects nuclear lamins and nuclear morphology

Year: 2026

Type: article

Abstract: Vesicle-associated membrane protein-associated protein A (VAPA) is a protein of the endoplasmic reticulum (ER) and a component of several membrane contact sites (MCSs). We show here that VAPA also localizes to the inner nuclear membrane (INM), in close proximity to nuclear lamins, INM proteins and nucleoporins. Using our proteomics approach 'rapamycin- and APEX-dependent identification of proteins by SILAC' (RAPIDS), we identified several nuclear proximity partners of VAPA, including emerin, different LAP2 isoforms, lamin A/C and Nup153. Depletion of VAPA in various cellular systems resulted in reduced nuclear lamin levels and aberrant nuclear morphology, including the formation of membrane invaginations and tunnels. Furthermore, histone acetylation levels were altered. Our data suggest that VAPA has distinct nuclear functions, in addition to its established role as an ER organizer.

Source: Journal of Cell Science

DOI: 10.1242/jcs.264298

Authors: Inés Rodríguez-González, David Kohlhause, Christof Lenz, Henning Urlaub, Christiane Spillner, Ralph H. Kehlenbach

Institutions: University of Göttingen, Universitätsmedizin Göttingen, Max Planck Institute for Multidisciplinary Sciences

Language: English

Topic: Nuclear Structure and Function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Deutsche Forschungsgemeinschaft

Award: G464257056