Year: 2025
Type: preprint
Abstract: This ELISA-based protocol confirms the identity and reactivity ofNonhuman Primate Reagent Resource (NHPRR) polyclonal goat anti-rhesus IgAand monoclonalmouse anti-rhesus IgAantibody reagents. BothIndirectandDirect ELISAformats are used to evaluate antibody binding specificity and lot-to-lot consistency. The procedure involves plate coating, blocking, sample incubation, detection, and data analysis, all performed according to defined acceptance criteria. Objective: To provide a standardized ELISA method for verifying the antigen specificity and identity of NHPRR anti-IgA antibodies. Scope: Applies to NHPRR laboratory staff performing antibody identity testing for research and quality control purposes. Background: Accurate identification of anti-IgA antibodies ensures reliable use of NHPRR reagents in immunoassays. TheIndirect ELISAdetects antibody binding through a secondary conjugate, while theDirect ELISAuses labeled anti-IgA for direct detection. Together, they confirm antibody specificity and support NHPRR’s quality testing program.
Institution: University of Massachusetts Chan Medical School
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7124315987
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