Work

Mapping metabolism using stable isotope tracers in combination with mass spectrometry imaging

Year: 2026

Type: article

Abstract: Stable isotope tracing combined with mass spectrometry imaging (MSI) offers a powerful framework for spatially and temporally resolved analysis of metabolic activity, enabling visualization of labeled metabolites at cellular and subcellular resolution. This approach provides insights into the spatial organization and dynamics of metabolic pathways such as glycolysis, the tricarboxylic acid cycle, and amino acid metabolism under both physiological and pathological conditions. However, its implementation remains technically challenging due to spectral complexity, limited spatial quantification methods, and the need for meticulous sample preparation and data processing. This review defines key considerations for integrating isotope tracers with MSI, including tracer selection, administration strategies across biological model systems, and current MSI platform capabilities. It also covers interpretation of isotopic labeling patterns and emerging experimental designs for mapping metabolic activity. By consolidating recent advances and identifying ongoing challenges, this review outlines the evolving role of MSI-based isotope tracing in spatial metabolomics.

Source: TrAC Trends in Analytical Chemistry

DOI: 10.1016/j.trac.2026.118679

Authors: Sarantos Kostidis, Hannah Mörk, Ton J. Rabelink, Martin Giera, Nina Ogrinc

Institutions: Leiden University Medical Center, Leiden University

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $5,150

Funders: Novo Nordisk, Novo Nordisk Fonden, European Research Council