Work

Tissue-derived extracellular vesicles: comparing Ts-EVs and Te-EVs in extraction, characteristics and research trends

Year: 2026

Type: article

Abstract: Extracellular vesicles (EVs) are lipid bilayer-enclosed nanovesicles that mediate intercellular communication by transferring bioactive molecules such as proteins, lipids, and nucleic acids. Tissue-derived EVs (Ti-EVs) have gained increasing attention due to their ability to reflect the physiological or pathological state of the tissue microenvironment. Ti-EVs are generally classified into two types: tissue-sourced EVs (Ts-EVs) and tissue-explant-derived EVs (Te-EVs). Although both types originate from tissues, they differ in isolation methods, biological characteristics, and research applications. This review systematically compares the isolation procedures, methodological advantages and limitations, and research progress of Ts-EVs and Te-EVs. Furthermore, we summarize their applications across various organ systems, focusing on their protein and RNA cargo. Finally, we discuss current challenges and future directions in Ti-EVs research.

Source: Cancer Cell International

DOI: 10.1186/s12935-026-04178-6

Authors: Junxia Xue, Defa Huang, Huangjie Zhou, Tao Qin, Yingqi Liu, Jie Chen

Institutions: First Affiliated Hospital of Gannan Medical University, The Affiliated Yongchuan Hospital of Chongqing Medical University

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,190