Work

Redirecting linear hydrocarbon metabolism toward polyhydroxyalkanoate biosynthesis

Year: 2026

Type: article

Abstract: This study explores how Pseudomonas putida EM2-4 metabolizes linear alkanes after acquiring an integrative and conjugative element (ICE) encoding a novel alkane monooxygenase (AlkB). This AlkB enzyme is a fusion of a hydroxylase and a fatty acid desaturase, exhibiting a narrow substrate range limited to n-octane and n-decane. The oxidation of these hydrocarbons yields octanoate and decanoate, respectively, which then enter the β-oxidation pathway and the glyoxylate shunt. Under nitrogen-limiting conditions, excess carbon is redirected toward polyhydroxyalkanoate (PHA) synthesis, a phenomenon particularly pronounced in a phaZ-deficient mutant unable to depolymeraze PHA, leading to up 75% of cell dry weight. Analysis of the PHAs monomer composition revealed variations with the carbon source. Additionally, 13C-isotopic labeling uncovered a minor but unexpected fraction of C10 monomers from C8 substrates, potentially arised from gluconeogenesis followed by the de novo synthesis of fatty acids. Proteomic analyses showed that the carbon source influences the activation of metabolic pathways, correlating with the observed variations in PHAs monomer composition.

Source: Microbial Cell Factories

DOI: 10.1186/s12934-025-02914-7

Authors: Rocío Palacios-Ferrer, María T. Manoli, Patricia Godoy, Antonio Delgado, Auxiliadora Prieto, Juan L. Ramos

Institutions: Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Centro de Investigaciones Biológicas Margarita Salas, Instituto Andaluz de Ciencias de la Tierra

Language: English

Topic: biodegradable polymer synthesis and properties

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $3,590

Funder: Consejo Superior de Investigaciones Científicas