Work

Protocol for minimizing off-target neuronal activation during optical stimulation in vivo

Year: 2026

Type: article

Abstract: In vivo optogenetic stimulation is a powerful approach for dissecting neuronal circuit function, yet it can inadvertently lead to activation of endogenous retinal opsins, producing off-target responses that propagate to the cortex and therefore confound experimental interpretation. Here, we present a protocol for optical device implantation in the mouse brain that minimizes light leakage from the implant and skull. We also describe in vivo electrophysiological techniques to assay off-target neuronal activity and ensure its amelioration. For complete details on the use and execution of this protocol, please refer to Weiler et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104342

Authors: Simon Weiler, Mateo Vélez-Fort, Luke O’Hara, Stephen C. Lenzi, Eleni Maria Amaniti, Troy W. Margrie

Institutions: Sainsbury Wellcome Centre, University College London

Language: English

Topic: Photoreceptor and optogenetics research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550