Work

Curcuma longa debranched starch assisted synthesis of cerium oxide nanoparticles and its antioxidant, anticancer, antimicrobial, and anti-biofilm activities

Year: 2026

Type: article

Abstract: Nowadays, there is a growing emphasis on eco-friendly methods for synthesizing metal nanoparticles (NPs), that avoid chemical processes and the production of harmful substances. In this study, we present a novel green approach for preparing cerium oxide nanodots (CeO 2 NPs) using Curcuma longa debranched starch as a reducing and capping agent via the sol-gel method. The prepared CeO 2 NPs were characterized using various techniques, including UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and zeta potential (ζ). An absorption peak of the particles at 315 nm was detected in the UV-Vis spectrum. Spherical CeO 2 NPs were observed by SEM and TEM with a 2–4 nm NP range. Energy-dispersive X-ray spectroscopy (EDX) analysis showed that the Ce, oxygen and carbon contents were 68.33 wt%, 25.59 wt and 6.07 wt%, respectively. The CeO 2 NPs exhibited antioxidant activity with an IC 50 value of 3.2 ± 0.23 µg/mL, and 3.66 ± 0.18 µg/mL for DPPH and ABTS free radical scavenging assays, respectively. Furthermore, CeO 2 NPs exhibited antibacterial activity against Corynebacterium diphtheriae , Klebsiella pneumoniae , Salmonella typhi , and Escherichia coli and antibiofilm activity against Corynebacterium diphtheriae and Escherichia coli. The in vitro cytotoxicity of CeO 2 NPs against HepG2 cells (IC 50 of 178 ± 14 µg/mL) was dose-dependent as evaluated using the MTT assay. These results collectively suggest the diverse functions of CeO 2 NPs, showcasing their potential as antioxidants, anticancer and antibacterial agents, and their usefulness in biomedical applications.

Source: Scientific Reports

DOI: 10.1038/s41598-026-35249-3