Accurate quantification of anti-drug antibodies against each unique Fab of a bispecific antibody using an ECL bridging assay
Year: 2025
Type: article
Abstract: AIM: To develop an anti-drug antibody (ADA) assay for a bispecific antibody (bsAb) we will refer to as Drug-1, and to quantify the ADAs against each specific fragment antigen-binding (Fab) site of Drug-1. MATERIALS AND METHODS: Here we use two additional unlabeled monoclonal antibodies (mAbs) Drug-2 & Drug-3 in an ADA confirmation assay format. Drugs-2 & -3 have the same targets as the two Fabs of Drug-1, facilitating our aim to measure and quantify the anti-drug Abs against each specific Fab of Drug-1 by signal inhibition. RESULTS AND CONCLUSION: By using the combination of a screening and a dual confirmation assay format, we were able to accurately quantify the ADAs against each specific Fab of the bsAb Drug-1.
Source: Bioanalysis
Institution: GlaxoSmithKline (United Kingdom)
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7124792338