Year: 2026
Type: article
Abstract: Organ-on-chip (OoC) systems are advancing rapidly as physiologically relevant in vitro models. However, real-time, noninvasive metabolic monitoring tools remain lacking. While NMR offers a powerful solution, current setups are not compatible with the planar format of microfluidic chips. Here we introduce the first benchtop NMR spectrometer for real-time metabolic monitoring of cell cultures on microfluidic platforms, utilizing hyperpolarization via dissolution dynamic nuclear polarization. This work details modifications made to a commercial benchtop NMR spectrometer, including the design and fabrication of a microfluidic platform that enables precise injection of hyperpolarized substrates and continuous renewal of cell culture media. The platform integrates a radiofrequency coil for signal transmission and reception and incorporates a custom-built sample carrier. Preliminary NMR data acquired with this system demonstrate its feasibility for dynamic metabolic studies.
Source: Analytical Chemistry
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Funders: “la Caixa” Foundation, Barcelona Institute of Science and Technology, European Commission, Ministerio de Ciencia e Innovación, Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina, European Regional Development Fund, Agencia Estatal de Investigación, European Social Fund, H2020 European Research Council, H2020 Future and Emerging Technologies, NextGenerationEU
Awards: G1175115578, G1204740081, G120905174, G121809054, G1272927172, G1401168639, G1622782223, G1855990336, G187364253, G210041235, G2262748287, G2309174360, G2601397997, G2660406951, G279363373, G280048558, G300979063, G34346518, G343855896, G3480869486, G348919436, G3518538256, G357246561, G363809202, G3672856370, G3681454997, G4042783231, G410571670, G425668148, G430777504, G4442677895, G451917667, G4526231891, G464105488, G5080475149, G5195188938, G531036588, G5421974903, G546199279, G5539308778, G5967599077, G6033112984, G6218905885, G6748603227, BENCHTOP NMR FOR LAB-ON-CHIP, G7084143925, G8015627718, G8277162176, G8546142835, G8805221138, G8841588148, G8888022036, G897484643, G929308097, G99296377
OpenAlex ID: W7124827237