Year: 2026
Type: article
Abstract: Gas vesicles (GVs) are air-filled protein nanoparticles which are proving to be useful in a number of biomedical applications. We hypothesized that it could be possible to develop a modular method for creating rapidly prototyped GVs by modifying their surface chemistry to include targeting peptides in an orientation-specific manner. Here, we describe a modular method to create targeted GVs using His-tagged antibody fragments, ensuring that the antibody fragments are connected to the GV in an orientation-specific manner. This is achieved via the functionalization of the GVs with nickel-nitrilotriacetic acid (Ni-NTA) group. First, we validated that these functionalized GVs can bind His-tagged green fluorescent protein and characterized the particle size and surface charge of functionalized GVs. Then, GVs targeted to prostate-specific membrane antigen (PSMA) using a minibody were validated using a knockout validation in vitro .
Source: Bioconjugate Chemistry
Authors: Reid Vassallo, Bill Ling, Ernesto Criado-Hidalgo, Nicole Robinson, Erik Schrunk, Ann Liu, George Daghlian, Hongyi R. Li, Margaret B. Swift, Dhiraj Mannar, Dina Malounda, S. Larry Goldenberg, Septimiu E. Salcudean, Mikhail G. Shapiro, Peter C. Black, Michael E. Cox
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7124828422