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CHARACTERIZATION OF CONFORMATIONAL CHANGES OF EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) IN BULK SOLUTION AT ACID AND BASE pH USING FLORESCENCE MEASUREMENTS TECHNIQUE

Year: 2017
Type: article
Abstract: The conformational changes of extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different pH values in solution where studied using fluorescence spectroscopy. The EPS was labeled using 8-aminopyrene-1,3,6-trisulfonic acid, tri sodium salt (APTS). The interactions between APTS and EPS surface in dilute aqueous solution were monitored using fluorescence spectroscopy. Steady state, excited state and time-resolved anisotropy measurements (TRAMS) were used to provide information about the changes in conformational behavior of the APTS as they bind to EPS surfaces.
Language: English
Subfield: Food Science
Open Access status: diamond
OpenAlex ID: W3038044282
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