Work

Guideline for the In Vivo Immunomodulatory Activity Test of Polysaccharide Based on Cyclophosphamide‐Induced Mouse Model

Year: 2026

Type: article

Abstract: ABSTRACT The immune system serves as the primary defense mechanism against pathogens and plays a vital role in maintaining host homeostasis. In recent years, natural polysaccharides have emerged as promising immunomodulators due to their structural complexity and ability to regulate immune responses through multi‐target pathways. Unlike conventional synthetic drugs, these biomacromolecules offer low toxicity, superior biocompatibility, and bidirectional immunoregulation, making them attractive candidates for immune‐related therapies. However, establishing a robust and reproducible immunosuppressive animal model is essential for accurately evaluating their immunoregulatory efficacy. This study systematically investigates the critical parameters affecting immunosuppressive model establishment, including animal species, sex, immunosuppressant type and dosage, administration frequency, and model termination timing. By optimizing these factors, we propose a standardized in vivo evaluation system for assessing the immunoregulatory activity of polysaccharides. This framework provides a scientifically rigorous approach to studying natural immunomodulators, facilitating their development into effective therapeutic agents.

Source: Food Safety and Health

DOI: 10.1002/fsh3.70076

Authors: Wenlei Nie, Xuecong Tong, Qiao Ding, Xiaojun Huang

Institutions: Nanchang University, Jiangxi Normal University

Language: English

Topic: Polysaccharides Composition and Applications

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,000