Explicit correction of severely non-uniform distributions of cryo-EM views
Year: 2026
Type: article
Abstract: The quality of three-dimensional macromolecular image reconstruction by cryo electron microscopy (cryo-EM) strongly depends on the number and the quality of the respective two-dimensional projections and on their angular distribution in space. Distributions with one or a few strongly preferred particle orientations may result in maps that are deformed in certain directions. A simple removal of overrepresented views may improve the quality of the reconstructed maps when the level of noise in the two-dimensional (2D) projections is low and the data-set size can afford this removal, but is counterproductive otherwise. Complementarily, giving an increased weight to underrepresented views, or taking multiple copies of them during the reconstruction, may improve the results, naturally, depending on how non-uniform the view distribution is. This work describes the results of three-dimensional (3D) reconstructions using an explicit correction of the number of overrepresented and underrepresented projections for non-uniformly distributed sets. Such correction can be considered as a potential preprocessing, fast and simple, during 3D reconstruction in the image-processing and cryo-EM structure-determination workflow.
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,230
Funders: Equipex, Agence Nationale de la Recherche, Institut National de la Santé et de la Recherche Médicale, Fondation pour la Recherche Médicale, Université de Strasbourg, Centre National de la Recherche Scientifique, Institut National Du Cancer, French Infrastructure for Integrated Structural Biology
Awards: G1190173965, Par-delà les frontières, l'Université de Strasbourg, G2255650664, G2290062404, G2659019386, G2694206344, Façonner les talents en formation et en recherche à l'Université de Strasbourg, G2980570810, G3357951378, G3730343, G399734812, G4399195447, Integrative Molecular and Cellular Biology, G471331628, G4779185514, G6136357505, G6803667113, G7240651115, Network of regulatory RNAs across kingdoms and dynamical responses to biotic and abiotic stresses., National instrumentation in cryo electron microscopy, Infrastructure Française pour la Biologie Structurale Intégrée
OpenAlex ID: W7125325342