Generation and characterization of iPSC models from HIV-1-positive individuals with divergent clinical outcomes
Year: 2026
Type: article
Abstract: The clinical outcome of human immunodeficiency virus type-1 (HIV-1) infection varies greatly among individuals, ranging from rapid disease progression to natural viral suppression. While viral and environmental factors contribute, host genetics are considered major determinants of disease trajectory. To enable mechanistic studies of host factors underlying disease outcomes, we generated 50 induced pluripotent stem cell (iPSC) lines from 18 participants of the Multicenter AIDS Cohort Study (MACS), spanning a spectrum of clinical trajectories. Reprogrammed MACS lines are confirmed to be HIV-1 negative and Sendai vector-free. We validate their pluripotency and demonstrate robust differentiation into macrophages capable of productive HIV-1 infection. These MACS-iPSC lines offer a genetically diverse resource to model HIV-1 infection in vitro, where clinical progression is known. Crucially, their capacity to differentiate into HIV-1 target cells and other disease-relevant lineages makes them a powerful tool to uncover host determinants of HIV-1 pathogenesis and advance targeted treatment and curative strategies.
Source: Stem Cell Reports
Institutions: King's College London, Northwestern University
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,190
Funders: GlaxoSmithKline, Wellcome Trust, King's College Hospital NHS Foundation Trust, NIHR Biomedical Research Centre, Royal Marsden NHS Foundation Trust/Institute of Cancer Research, National Institute for Health and Care Research, Department of Health and Social Care, King's College London, National Institutes of Health, Directorate for Biological Sciences, Medical Research Council, Biotechnology and Biological Sciences Research Council, National Institute of Allergy and Infectious Diseases
Awards: Using iPSC variation to define HIV-1 regulatory networks, King's College London MRC Doctoral Training Partnership in Biomedical Sciences: iCASE, G6868616705, Multicenter AIDS Cohort Study, Revectorisation of HIV 1 and lentiviral vector production in alternative host cell lines, Cell therapies and regenerative medicine., Interferon and Human Pandemic Viruses, G8872864032, G9000126231, G9000127601
OpenAlex ID: W7125367683