A Preliminary In Vitro Interaction of ET‐26‐HCl With Human Serum Albumin Through Rapid Equilibrium Dialysis Under Simulated Physiological Conditions
Year: 2026
Type: article
Abstract: ABSTRACT In the present study, preliminary in vitro interaction between ET‐26‐HCl and human serum albumin (HSA) was investigated, and the potential competitive binding effect from its major metabolite, ET‐26‐acid, was evaluated. Rapid equilibrium dialysis (RED) was utilized under simulated physiological conditions (67 mM phosphate‐buffered saline solution, pH 7.4, 37 °C). After a methanol‐based deproteinization procedure, a validated HPLC‐UV method was employed to quantify post‐dialysis samples. It was found that the binding affinity of ET‐26‐HCl to HSA was relatively lower than that of etomidate, with a binding association constant ( K ) of 7.75 × 10 3 M −1 and a protein binding rate ( PB %) of 54.58%, respectively. Displacement experiments using warfarin and flurbiprofen as specific site markers revealed that ET‐26‐HCl predominantly binds to Sudlow's Site I on HSA. Furthermore, ET‐26‐acid was found to exhibit no significant competitive binding effect. These findings support ET‐26‐HCl as a promising alternative to etomidate and warrant its further development for clinical anesthetic applications.
Source: Biomedical Chromatography
DOI: 10.1002/bmc.70362
Authors: Wen You, Yifei Bian, Yuze Ji, Ling Sun, Lili Xiang, Dongying Chen
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7125393402