Work

Biosynthesis of Chlorphenesin Galactoside and Comparative Analysis of HPLC with Chlorphenesin

Year: 2025

Type: article

Abstract: A common cosmetic preservative, chlorphenesin (CPN), was enzymatically modified by galactosylation to improve its physical and chemical properties. In the present study, CPN and CPN galactosidase completed a 48-hour reaction. Thin-layer chromatography (TLC) was first used to monitor the galactosylation process. The new product was further analyzed by using high-performance liquid chromatography (HPLC). The HPLC chromatogram for standard CPN showed a much longer retention time (RT), whereas CPN-gal eluted earlier, due to increased polarity and galactose conjugation. The difference in retention period between CPN and CPN-gal confirms the successful enzymatic galactosylation. Overall, on the basis of results CPN-gal preservatives might be suitable alternate for safer cosmetic uses.

Source: Journal of Biotechnology and Bioindustry

DOI: 10.37503/jbb.2025.13.26

Authors: Jae Hyeob Kim, Hyang-Yeol Lee

Institution: Korea National University of Transportation

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: Korea National University of Transportation

Award: G3420669131