Effect of dimethyl sulfoxide pretreatment on bonding of universal adhesives to pulp chamber dentin
Year: 2026
Type: article
Abstract: Aim and objective: To evaluate the effect of 50 % (v/v) dimethyl sulfoxide (DMSO) pretreatment on immediate (24 h) and delayed (6 months) microtensile bond strength (μTBS) of an etch-and-rinse adhesive and two universal adhesives to dentin. Materials and methods: A total of 144 extracted human molars were decoronated, pulp chambers exposed, and roots sectioned 2 mm apical to the CEJ. Teeth were randomly divided into six experimental groups (n = 24), based on surface pretreatment and adhesive system used: Group 1A: Adper Single Bond 2 (ASB) Group 2A: Tetric N-Bond Universal (TNBU) Group 3A: Prime & Bond Universal (PNBU) Groups 1B, 2B, and 3B received the same adhesives, respectively, following a 50 % (v/v) DMSO wet bonding pretreatment. All adhesives were applied per manufacturer's instructions, followed by composite resin build-up. Ten samples per group were tested for μTBS at 24 h and ten after 6 months of storage. Failure mode was analyzed. Four additional samples per group underwent SEM evaluation to observe the resin-dentin interface. Statistical analysis: Data were analyzed using one-way ANOVA and Tukey's post hoc test for intergroup comparisons. Unpaired t-tests were applied for two-group comparisons. Significance was set at p < 0.05. Results: DMSO pretreatment significantly improved both immediate and long-term μTBS (p < 0.05) across all adhesives. PNBU exhibited the highest bond strength at both time intervals. Conclusion: Dentin pretreatment with 50 % DMSO prior to adhesive application enhances immediate μTBS and preserves bond durability at 6 months, regardless of adhesive system used.
Institution: Kothiwal Dental College and Research Centre
Language: English
Subfield: Orthodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,800
Award: G1387897576
OpenAlex ID: W7125933371