Work

A surrogate‐selection‐enhanced TnpBmax platform enables efficient genome editing in rice and tomato

Year: 2026

Type: article

Abstract: TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrogate (TnpBmax) enriches edited cells, achieving up to 81.5% efficiency and high homozygosity across rice and tomato.

Source: Journal of Integrative Plant Biology

DOI: 10.1111/jipb.70159

Authors: Enze Li, Jiyong Xie, Xinbo Li, Zai Zheng, Mugui Wang, Jian‐Kang Zhu, Yifu Tian

Institutions: Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Institute of Crop Sciences, Chinese Academy of Tropical Agricultural Sciences, Southern University of Science and Technology, South China Agricultural University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funders: National Natural Science Foundation of China, China Postdoctoral Science Foundation, National Postdoctoral Program for Innovative Talents, National Key Research and Development Program of China

Awards: G1188758147, G213605365, G2556892354, G3420946383

OpenAlex ID: W7126017017

FWCI: 5.816

Cites: 10

Cited by: 3

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