Year: 2026
Type: article
Abstract: Harmful algal blooms pose significant risks to water resource management and aquatic ecosystem health, rendering early detection of algal bloom proliferation essential. In this study, we present an electrochemical strategy for the real-time detection of individual Chlorella cells using the single-particle collision method at an ultramicroelectrode (UME). The detection principle relies on monitoring changes in the redox probe flux at the UME induced by attachment of the target. Both diffusional and migrational transport were considered to promote particle collision at the UME. Detection sensitivity for negatively charged microalgae was enhanced by exploiting migration effects. To control migration strength, neutral and charged redox probes were selected, and the ionic strength was adjusted to tune electrostatic attraction, yielding microalgae capture on the UME with a collision frequency that depended on the solution composition. Conversely, migration was suppressed by increasing the ionic strength, and inverse migration was implemented, and resulting collision responses were compared. Furthermore, COMSOL Multiphysics simulations were used to estimate the size of detected Chlorella cells. The collision frequencies expected from diffusion and migration were compared with the experimental values, and a calibration curve relating collision frequency to Chlorella concentration was established. Consequently, this methodology provides a promising platform for the early monitoring of algal blooms by simultaneously determining microalgal size and concentration.
Source: Sensors
DOI: 10.3390/s26030915
Authors: Changhui Lee, Gayeon Lee, Jun Hui Park
Institution: Chungbuk National University
Language: English
Subfield: Electrochemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,137
Award: G7840991751
OpenAlex ID: W7126237344