Work

Soaking Up Success: Sponge-Assisted Nanoparticle Transfection

Year: 2026

Type: preprint

Abstract: Current cell transfection systems face efficiency limitations, particularly for challenging cell types. We present a sponge-assisted transfection method enabling efficient mRNA transfection at nanogram-scale doses—a several orders of magnitude-reduction compared to the microgram quantities typically required by lipid-based and membrane-disruption methods. This approach achieves robust, dose-dependent transfection across difficult-to-transfect cell types, establishing broad applicability for both basic research and therapeutic use.

Source: Research Square

DOI: 10.21203/rs.3.rs-8428323/v1

Authors: Yevgeny Brudno, Rukesh Chinthapatla, Hayden Sandt, Israt Jahan Tulip, Vedamudra Kolluru, Hannah Haynes, Savannah Muron, Sharda Pandit, Christopher Moody, Madelyn VanBlunk, Soumen Saha, Ashutosh Chilkoti, Erin Heinzen, Pritha Agarwalla

Institutions: University of North Carolina at Chapel Hill, Duke University

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: North Carolina State University, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

Awards: G2262217435, G4741720324, G7366156696, G7998276848, G9000033364, Biomaterial Scaffolds for Ex Vivo and In Situ CAR-T Cell Production, MASTER Scaffolds for Rapid, Single-Step Manufacture and Prototyping of CAR-T cells, Bioresorbable Polythioketal Urethane Wound Dressings, NRSA in Genetics, Cancer Center Support Grant, G958207224

OpenAlex ID: W7126239851

Cites: 14

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Related to: 10