Year: 2026
Type: article
Abstract: Dietary bioactive compounds are increasingly explored as complementary cardioprotective strategies, and the nitration of unsaturated fatty acids has emerged as a process capable of enhancing antiplatelet properties. This study investigated whether Phaseolus vulgaris L. extracts can generate nitrated fatty acids under gastric-like conditions and evaluated their effects on human platelet function. Bean extracts and major fatty acids were nitrated in vitro and tested using washed platelets to assess cytotoxicity, TRAP-6 and collagen-induced aggregation, activation markers (P-selectin, CD63), and mitochondrial responses including membrane potential, ROS production, and Ca2+ dynamics. Nitrated extracts markedly inhibited TRAP-6 induced aggregation (IC50 ≈ 1.8 mg/mL), whereas non-nitrated extracts showed minimal activity; this effect was reversed by β-mercaptoethanol, indicating dependence on electrophilic nitroalkenes. Fractionation revealed that the lipidic fraction accounted for most of the antiplatelet effect, and isolated nitrated fatty acids (NO2-LN, NO2-LA, NO2-OA) displayed stronger inhibition than their native counterparts without increasing cytotoxicity. Nitrated species additionally reduced mitochondrial membrane potential and granule secretion without elevating ROS. These findings identify Phaseolus vulgaris L. as a natural source of bioactive nitrated fatty acids and support their potential as nutraceutical agents capable of modulating platelet activation and contributing to cardiovascular risk reduction.
Source: Molecules
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,258
Awards: SYNTHESIS OF ORTHO-CARBONYL HYDROQUINONE DERIVATIVES THAT BLOCK MITOCHONDRIAL BIOENERGETICS AS A NOVEL ANTIPLATELET STRATEGY FOR THROMBOSIS PREVENTION WITHOUT INCREASING BLEEDING RISK., RED INTERNACIONAL PARA LA PREVENCION DEL SINDROME GERIATRICO DE FRAGILIDAD EN PERSONAS MAYORES, "RED INTERNACIONAL PARA POTENCIAR EL EFECTO CARDIOPROTECTOR DE PHASEOLUS VULGARIS L., A TRAVES DE LA NITRACION DE SUS ACIDOS GRASOS"., UNIDAD DE BIOENERGETICA MITOCONDRIAL PARA LA INVESTIGACION TRANSDISCIPLINARIA Y TRASLACIONAL DESDE MODELOS CELULARES A LA CLINICA, MULTIDISCIPLINARY STUDIES ON BIOACTIVE COMPOUNDS FROM THE CHILEAN PHASEOLUS VULGARIS L. LANDRACES AND THEIR POTENTIAL EFFECTS IN THE PREVENTION OF NON-COMMUNICABLE CHRONIC DISEASES, UNIDAD MULTIDISCIPLINARIA DE CITOMETRIA DE FLUJO PARA EL ANALISIS DE PARTICULAS Y POBLACIONES CELULARES INVOLUCRADAS EN ENFERMEDADES CRONICAS DEL ENVEJECIMIENTO
OpenAlex ID: W7126245940