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Hplc Analytical Method Development and Validation of Anti-tubercular Drugs: a Comprehensive Review

Year: 2026

Type: article

Abstract: Tuberculosis (TB) remains a major global health challenge, necessitating highly reliable analytical methods for the quantification of first-line and second-line anti-tubercular drugs (ATDs) in pharmaceuticals and biological matrices. High-Performance Liquid Chromatography (HPLC) continues to be the most widely applied technique for routine analysis due to its selectivity, sensitivity, and robustness. This review provides an extensive discussion on chromatographic principles, method development strategies, validation parameters, and analytical challenges associated with ATDs such as isoniazid, rifampicin, pyrazinamide, ethambutol, fluoroquinolones, linezolid, Bedaquiline, Delamanid, and Pretomanid. Detailed literature summaries, forced degradation considerations, and future prospects of green chromatography, AQbD, and hyphenated techniques are highlighted.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.18426963

Author: Gali Haritha1*, Shaik Naziya Fathima2, Arunabha Mallik3

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7126256452

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