Work

Systematic Review of HPLC Methods Using UV Detection for Quantification of Vitamin E in Human Plasma

Year: 2026

Type: review

Abstract: Measurement of vitamin E levels is used to evaluate the health status in humans. For routine analytics in clinical laboratories, an accurate, quick, and simple determination method is required. An option for the quantification of vitamin E (α-tocopherol) in human blood samples is the use of high-performance liquid chromatography (HPLC) in combination with a UV detector. Several sample preparation methods for this purpose have been reported in the literature. Our aim was to generate an overview and comparison of the different methods. The online database PubMed was searched for published HPLC methods. Of 77 reports screened, 16 methods were selected and summarized in tables. These present the parameters of the sample preparation procedure, HPLC settings, and some validation criteria (limit of detection (LOD), limit of quantification (LOQ), and intra- and inter-assay values, recovery rates) of the reported methods. In the frame of our methodological review, we could find some extraction approaches. The liquid–liquid extraction with hexane or the double extraction with hexane were often used. Another possibility is the single extraction approach. This systematic review highlights the similarities and differences in methods, and it can therefore be used to develop and establish methods in a laboratory.

Source: LabMed

DOI: 10.3390/labmed3010004

Authors: Miriam Demtschuk, Priska Heinz

Institution: Private University in the Principality of Liechtenstein

Language: English

Topic: Antioxidant Activity and Oxidative Stress

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,207