Work

Optimization of agarose–gelatin double embedding to minimize sectioning artifacts in multicellular spheroids

Year: 2026

Type: article

Abstract: Processing methods that do not induce shape distortion are essential for the analysis of tissue morphology. Although the use of agarose-gelatin double embedding to reduce shape distortion has been suggested, the effect of matrix concentration has not been addressed. Therefore, combinations of agarose (1-3%) and gelatin (1-10%) were evaluated using multicellular spheroids without extracellular matrix as a model of mechanical fragility. In this study, a blend of 2% agarose and 5% gelatin effectively prevented distortion. This protocol can enhance the morphological analysis of delicate tissues.

Source: BioTechniques

DOI: 10.1080/07366205.2026.2621053

Authors: Hyoyoung Maeng, Min-Gi Han, Yoseop Jeon, Donghyeon Kim, Yuna Park, Hyuk Song

Institution: Konkuk University

Language: English

Topic: Medical and Biological Sciences

Subfield: Anatomy

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funder: National Research Foundation of Korea