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A Single Residue Switch Controls Isomer Selectivity in the Myxobacterial Isobonnadiene Synthase

Year: 2026
Type: article
Abstract: Myxobacterial terpenoids are rare and only a few myxobacterial terpene synthases are known. A terpene synthase, IbdS, from Polyangium aurulentum sp. SDU3-1 was found to produce the [delta]9,10 isomer (1) of the spirocyclic 6/7/5-tricyclic bonnadiene, along with several 6/10-eunicellanes and cembrenoids as minor products. Sequence comparison and site-directed mutagenesis of IbdS and the actinobacterial bonnadiene synthase revealed a single residue switch at position 57 controlling isomer selectivity.
DOI: 10.1021/acs.orglett.6c00100
Language: English
Open Access status: green
Funders: U.S. Department of Energy, University of Florida, Joint Genome Institute, National Institutes of Health, Office of Science, NIH Office of the Director, National Institute of General Medical Sciences
Awards: G1286236842, Acquisition of a Q Exactive HF Hybrid Quadrupole-Orbitrap, G3083819904, G3146530874, G378104201, G382641700, G419881185, G42916606, G4501827968, G498139845, G7205239661, G789161557, G834277795, G9000086347, G969889393
OpenAlex ID: W7127312481
FWCI:
Cites: 32
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