Year: 2026
Type: article
Abstract: Myocardial infarction (MI) triggers complex pathological processes, including inflammation, hypoxia, and fibrotic remodeling. MicroRNAs (miRNAs) have emerged as promising biomarkers for cardiovascular injury; however, their expression dynamics along processes remain underexplored. We used an in vivo rat model of permanent coronary occlusion to study the molecular alterations associated with MI and its resolution in a temporal mode, including five experimental groups with five animals in each: sham, PO 24 h, PO 72 h, PO 7 d, PO 1 month. Histological analysis, serum biomarkers, and miRNA/gene expression profiles were analyzed in a time-dependent manner post-occlusion. Subsequent analysis revealed early depletion of selected circulating miRNAs (PO 24 h). Transient upregulation in cardiac tissue miRNAs, inflammatory and fibrotic gene expression (Fibronectin, Collagen, Vimentin, E-Cadherin) were observed at PO 72 h. These molecular alterations correlated with histological evidence of myocardial injury and repair. Taken together, our findings delineate the molecular timeline of MI progression and resolution and identify candidate miRNAs as sensitive and time-dependent indicators of myocardial stress, including miR-107, miR-122-5p and miR-221-3p. This integrative approach supports the use of miRNA signatures for noninvasive monitoring of cardiac injury and resolution and unveils potential therapeutic targets to reduce pathological remodeling.
DOI: 10.3390/ijms27031488
Authors: Macarena Rodríguez-Serrano, Elena Martín-García, Patricia Alonso-Andrés, Elisa Conde-Moreno, Héctor Pian, Javier del Moral-Salmoral, Nunzio Alcharani, Miriam Menacho-Román, Lorena Crespo-Toro, Miren Edurne Ramos-Muñoz, Carlos Zaragoza, Luis Miguel Rincón, María G. Barderas, María Laura García-Bermejo
Institutions: Instituto Ramón y Cajal de Investigación Sanitaria, European Infrastructure for Translational Medicine, Universidad Francisco de Vitoria, Instituto de Salud Carlos III, Centro de Investigación en Red en Enfermedades Cardiovasculares, Centro de Investigación Biomédica en Red, Hospital Universitario Ramón y Cajal, Universidad de Salamanca, Instituto de Investigación Biomédica de Salamanca, Servicio de Salud de Castilla La Mancha, Hospital Nacional de Parapléjicos
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,499
Funder: Instituto de Salud Carlos III
Awards: Deep learning para la predicción de muerte y eventos cardiovasculares con tomografía computarizada – generación y validación de un modelo IA (CAPTIVATE), G1619182958, G2854544501, miRNAs como mediadores dinámicos del proceso de EMT y TEM en el desarrollo y progreso del tumor renal de células claras: su utilidad como biomarcadores y potenciales dianas terapéuticas, Implicación de largos RNAs no codificantes(lncRNAs) en el carcinoma renal de células claras avanzado: estatificando de forma precisa a los pacientes para tratamientos con imunoterapia y antiangiogénicos.
OpenAlex ID: W7127317094