Work

Comparison between in-house competitive and commercial enzyme-linked immunosorbent assays for the detection of heat shock protein 70 in milk, blood, and saliva of dairy cows

Year: 2026

Type: article

Abstract: values of 0.777, 0.833, and 0.752 for milk, blood, and saliva, respectively. The findings indicate that the in-house ELISA closely aligns with the performance of the commercial kit. Despite the lower detectable minimum concentration of the commercial assay, the in-house assay provides a more economical alternative for evaluating physiologically high and moderate HSP70 levels; however, the commercial kit remains widely available for purchase. Among biological matrices, plasma exhibited the highest cross-assay correlation, whereas milk and saliva showed consistent relative trends, indicating their potential as noninvasive alternatives for HS monitoring in dairy cows.

Source: Journal of Dairy Science

DOI: 10.3168/jds.2025-27811

Authors: M.R.H. Rakib, V. Messina, J.I. Gargiulo, I.N. Pathirana, N.A. Lyons, S.C. Garcia