Work

Bioinspired artificial nanovesicles engineered from 3D spheroid-cultured UC-MSCs enhance angiogenic activity in vitro: functional proof-of-concept for ischemic applications

Year: 2026

Type: article

Abstract: Artificial nanovesicles (aNVs) derived from cells may mimic naturally secreted extracellular vesicles (EVs) and are becoming popular in biomedical research. We isolated aNVs from two-dimensional (2D)- and three-dimensional (3D)-cultured umbilical cord-derived mesenchymal stem cells (UC-MSCs) (aNVs 2D and aNVs 3D , respectively) and characterized them using Western blotting and electron microscopy. The aNVs 3D showed higher expression of IL-6 and SDF-1α than aNVs 2D . In vitro treatment with aNVs 2D and aNVs 3D resulted in their internalization into endothelial cells and the subsequent alteration of endothelial cell proliferation, migration, and tube formation. Both aNVs were positive for EVs and cell markers and were round in shape. Furthermore, aNVs 3D treatment enhanced endothelial cell proliferation, migration, and tube formation more effectively than aNVs 2D treatment. Our study demonstrates that aNVs3D are potent inducers of angiogenesis, indicating their potential in cell-free ischemia treatment.

Source: Frontiers in Cell and Developmental Biology

DOI: 10.3389/fcell.2026.1695559

Authors: Prakash Gangadaran, Ramya Lakshmi Rajendran, Ji Min Oh, Jaeyoung Son, Chae Moon Hong, Byeong-Cheol Ahn

Institutions: Kyungpook National University, Kyungpook National University Hospital

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801