Classical conditioning of Purkinje cell responses in vitro produces in vivo-like simple spike suppressions during the conditional stimulus
Year: 2026
Type: article
Abstract: An in vitro model of classical conditioning could improve our understanding of underlying mechanisms thanks to controlled pharmacological manipulation and negligeable perturbations from external networks. Here, using mice cerebellar slices and patch clamp recordings, we present a protocol for classical conditioning of Purkinje cells in vitro at room temperature. Repeated pairing of a parallel fiber conditional stimulus and a climbing fiber unconditional stimulus with parameters compatible with in vivo learning gives rise to a Purkinje cell simple spike suppression during the conditional stimulus that shares similarities with conditioned responses observed in live animals. • New approach to classical conditioning in vitro with parameters used in vivo . • Circumvents in vitro limitation, with precise control on concentration and delivery of pharmacological agent. • Blocking GIRK channels in vitro prevents learning similarly to in vivo studies.
Institution: Lund University
Language: English
Topic: Memory and Neural Mechanisms
Subfield: Cognitive Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,700
OpenAlex ID: W7127897360