Work

The Rostral Ventrolateral Medulla Input to Central Amygdala Regulates Anxiety‐Like Behaviors in Mice for Chronic Light Exposure

Year: 2026

Type: article

Abstract: BACKGROUND: Emerging evidence increasingly links environmental light exposure to the development of anxiety disorder. The rostral ventrolateral medulla (RVLM), a key brainstem center that regulates sympathetic outflow and blood pressure (BP), has been implicated in the autonomic dysregulation frequently observed in anxiety. High blood pressure is a recognized exacerbating factor for anxiety-related pathology. Although aberrant projections from the central amygdala (CeA) to brainstem regions have been reported in multiple mood disorders, it remains unclear whether the CeA receives input from the RVLM and to what extent the RVLM's excitation influences anxiety symptoms induced by chronic light exposure (CL). METHODS: We employed a chronic mild light (250-300 lx) for 4 weeks to induce anxiety-like behaviors in mice. Using a tail-cuff system and ELISA assay, we assessed CL mice's BP and plasma catecholamine levels. Immunofluorescence was utilized to unravel the neuronal c-fos expression in the RVLM and CeA. Combining retrograde virus tracing technology, chemogenetic and optogenetic manipulations in freely moving mice to examine the effects of regulating RVLM-CeA pathway on anxiety-like behaviors induced by chronic light. RESULTS: Chronic light exposure in mice induces elevated blood pressure and elevated neuronal activity, which are more pronounced in the RVLM than in the CeA. Chemogenetic inhibition of RVLM neurons markedly attenuated CL-induced anxiety-like behaviors. Moreover, optogenetic inhibition of the RVLM→CeA pathway reduced anxiety phenotypes in CL-exposed mice, whereas optogenetic activation of this pathway in normal mice acutely triggered anxiety-like behaviors. CONCLUSIONS: These findings demonstrate that the enhanced excitatory signaling within the RVLM→CeA circuit underlies the development of anxiety-like behaviors induced by chronic light, revealing a novel mechanism by which the RVLM regulates light-related affective dysfunction.

Source: CNS Neuroscience & Therapeutics

DOI: 10.1002/cns.70775

Authors: Jia‐Ni Jing, Xing Tan, Chou Xu, Chao Yuan, Hao Fan, Wei‐Zhong Wang

Institutions: Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences, Second Military Medical University, Chinese People's Liberation Army

Language: English

Topic: Stress Responses and Cortisol

Subfield: Behavioral Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $4,140