Antibacterial and Antibiofilm Efficacies of Cell-Free Supernatant of Dubosiella newyorkensis Against Pseudomonas fluorescens and Its Application in Food Systems
Year: 2026
Type: article
Abstract: Pseudomonas fluorescens is a common spoilage bacterium in aquatic products, posing a significant challenge to food safety due to its strong adhesion capacity and ability to form resilient biofilm on various surfaces. Effective strategies to inhibit its biofilm formation are urgently required. Dubosiella newyorkensis, a recently identified probiotic candidate, has attracted increasing interest for its unique physiological characteristics and potential health benefits. Recent studies have found that cell-free supernatants (CFS) of probiotics have a variety of effects. In this study, we explored the anti-biofilm potential of the CFS derived from D. newyorkensis against P. fluorescens. The CFS notably inhibited bacterial growth, impaired motility, reduced surface hydrophobicity and self-aggregation, and significantly suppressed biofilm formation. Additionally, compared with untreated bacteria, CFS-treated P. fluorescens decreased attachment to common food contact surfaces. RT-qPCR further revealed that CFS treatment significantly downregulated the expression of key biofilm-related genes. These findings suggest that D. newyorkensis CFS possesses both antibacterial and anti-biofilm properties against P. fluorescens. This study highlights the potential application of D. newyorkensis-derived CFS as a biopreservative for extending the shelf life of aquatic food products.
Source: Foods
Institution: Dalian Polytechnic University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,499
OpenAlex ID: W7127957195