Serum miR-381-3p: Diagnostic Role and Mechanisms in Liver Transplant Ischemia-reperfusion Injury
Year: 2026
Type: article
Study design: Observational Study
Abstract: Background and Aims: Hepatic ischemia-reperfusion (HIR) injury impairs outcomes post-liver transplantation.Therefore, we aimed to investigate the role and mechanism of miR-381-3p in HIR.Methods: The study enrolled 150 healthy controls, 82 non-HIR-injured patients, and 68 patients with HIR injury following liver transplantation.Clinical data were analyzed.Multivariate analysis identified HIR risk factors; the predictive value of miR-381-3p was assessed via receiver operating characteristic analysis.An in vitro hypoxia/ reoxygenation (H/R) model was established and employed.The cellular effects of miR-381-3p and JAK2 were evaluated using CCK-8, flow cytometry, ELISA, luciferase, RIP, and bioinformatics.Results: Serum miR-381-3p was significantly elevated in HIR compared with the other groups.miR-381-3p was the strongest independent HIR risk factor, which was confirmed by receiver operating characteristic analysis.H/R upregulated miR-381-3p.Inhibiting miR-381-3p counteracted H/R-induced decreased viability and increased apoptosis, inflammation, and oxidative stress.miR-381-3p directly bound to and suppressed JAK2 via its 3′ untranslated region (validated by luciferase and RIP).Transfection of si-JAK2 abolished the protective effects of miR-381-3p inhibition.Conclusions: miR-381-3p exacerbates post-transplant HIR by directly targeting JAK2, amplifying inflammation and oxidative stress.Thus, our findings nominate serum miR-381-3p as a promising non-invasive biomarker and suggest its potential as a therapeutic target for mitigating HIR injury.
Authors: Xiaobin Chi, Zerun Lin, Zhijian Chen, Jianda Yu, Yongbiao Chen, Honghuan Lin, Qiucheng Cai, Lizhi Lv
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7127991423
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